Daemen University is located on a 46.5-acre campus in Amherst, New York. The suburban location enables students of the university to have ready access to the vibrant Buffalo-Niagara region while enjoying the charm of a beautifully landscaped campus. Daemen is easily accessible by major rail, plane, and motor routes servicing Buffalo. The university is less than five minutes from exit 50 of the New York State Thruway. The Buffalo Niagara International Airport, serviced by most major airlines, is a 15-minute drive from the campus.
- Academic and Wellness Center (AWC), located across from Daemen’s Main Street campus, houses the fitness center and recreation space, as well as state-of-the-art classrooms for physical therapy, athletic training, and health promotion.
- Alumni House is the home for institutional advancement, alumni, and publication offices.
- Athletics Complex houses the Lumsden Gymnasium as well as the university's intercollegiate athletic offices and athletic training room.
- Business Building includes faculty offices, classrooms, a computer lab, and a student lounge, as well as the Office of Information Technology.
- Campus Apartments, housing approximately 384 students, is a complex of seven two-story apartment-style buildings, typically providing housing for upperclassmen students. Each apartment has four bedrooms, two bathrooms, a living room, and a kitchen. Laundry facilities are available on each floor.
- Canavan Hall is a five-story, suite-style residence hall, housing approximately 260 students, primarily used as first-year and new student housing. Two bedrooms share one bathroom. Lounges and laundry facilities are available on each floor.
- Charles J. Wick Campus Center houses student life offices including Campus Safety, CHIP Center (Counseling, Health, Insurance, Prevention), Conference Services, the 24/7 Information Desk, and the executive boardroom, a dedicated esports center, lounges, the main dining room seating 650, the Wildcat Den (multi-media dining location), and a social room/auditorium seating 500.
- Patricia E. Curtis Hall houses physician assistants, psychological sciences, and social work faculty offices.
- Duns Scotus Hall is the home of the SPOT student services corridor. DS houses administrative and faculty offices, classrooms, science laboratories, art studios, lounges, the bookstore, print shop, mailroom, the Nancy Haberman Gacioch Center for Veterans, Diversity and Multicultural Affairs, Global Programs (International Student Services & Study Aboard), Paul A. Saffrin Center for Sustainability & Civic Engagement (includes Service-Learning), and the Student-Athlete Academic Center.
- Haberman Gacioch Center for Visual & Performing Arts features the Tower Gallery, graphic design production area, computer labs, faculty offices, the Sister Jeanne File Resource Room, the animation center, and studios for illustration, figure drawing, and painting. The building was designed with green technology and uses geothermal heating to contain energy costs.
- Honors House, located near the university's Getzville Road entrance, serves as a study space for Honors Program students and provides office space for the marketing team.
- MusicalFare Theatre houses the theater and offices of MusicalFare Theatre, as well as campus event space.
- Research and Information Commons (RIC) was built with innovative green technology and are LEED Gold-certified. The RIC houses a comprehensive library, Academic Support Services, Disability Services, Graduate Studies Technology Center, the Professional Educator Resource Center, Smart Squad, 78 WEST cafe, small-group study rooms, a seminar and screening room with surround sound and 3D projection capabilities, and many state-of-the-art amenities to enhance the study experience.
- Rosary Hall is the original university administrative and classroom building, which was restored and houses the executive dining room and the Division of Enrollment Management & Admissions.
- Schenck Hall houses laboratory facilities, classrooms, and a 300-seat lecture hall.
- The Thomas Reynolds Center for Special Education & After-School Programs includes a conference room, observation room, and four state-of-the-art classrooms.